Volumes Archive
Vol. 45, Number 3, 2003
A Rapid and Sensitive HPLC Method for the Determination of Naproxen in Plasma
Bassam M. Tashtoush and Bashar M. AI-Taani
Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid -Jordan
Abstract
A sensitive, rapid method using an isocratic high-pressure liquid chromatography and flourometric detection for the determination of naproxen sodium in plasma is presented. Reverse phase micobondapack column was used with a mobile phase consisting of 42% acetonitrile and 58% water adjusted to pH 3 using phosphoric acid. The flourometric detector with an excitation wavelength of 270 nm and emission wavelength of 340 nm provided high sensitivity and no interferences from plasma constituents. Plasma samples were injected to HPLC without any extraction. The method was precise, reproducible as it was demonstrated by replicate analysis of pooled plasma sample containing 0.5 - 80 µg/mL naproxen sodium
Key words: Naproxen, HPLC, fluorescence detection, plasma.
